Columbia Broth Base, HiVeg with Blood

[所属分类:培养基配方] [发布时间:2020-5-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Columbia Broth Base, HiVeg with Blood

培养基配方
Composition per liter:
Plant peptone No.5.............................................................10.0g
Plant special peptone .........................................................10.0g
NaCl............................................................................5.0g
Plant infusion..................................................................3.0g
Tris(hydroxymethyl)aminomethane.................................................2.86
Glucose ........................................................................2.5g
Na2CO3 .........................................................................0.6g
L -Cystine hydrochloride........................................................0.1g
MgSO4 ..........................................................................0.1g
FeSO4 .........................................................................0.02g
Sheep blood, defibrinated.....................................................50.0mL
pH 7.5 ± 0.2 at 25°C
Source: This medium, without blood, is available as a premixed pow-
der from HiMedia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat until
boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Add blood and/or selective antimi-
crobics. Mix well.
Use: For the cultivation and isolation of fastidious bacteria from clin-
ical specimens.