Columbia Blood Agar Base with Horse Blood (LMG Medium 151)

[所属分类:培养基配方] [发布时间:2020-5-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Columbia Blood Agar Base with Horse Blood
(LMG Medium 151)

培养基配方
Composition per liter:
Columbia blood agar base ...............................................950.0mL
Horse blood..............................................................50.0mL
pH 7.3 ± 0.2 at 25°C
Columbia Blood Agar Base:
Composition per liter:
Special peptone..........................................................23.0g
Agar ....................................................................10.0g
NaCl......................................................................5.0g
Starch ...................................................................1.0g
Preparation of Columbia Blood Agar Base: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C.
Preparation of Medium: To 950.0mL of cooled, sterile Columbia
blood agar base, aseptically add 50.0mL of sterile, defibrinated horse
blood. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the cultivation of Arcanobacterium spp., Paenibacillus spp.,
Corynebacterium spp., Lactobacillus iners, Globicatella sanguinis,
Gemella morbillorum, Enterococcus cecorum, Enterococcus colum-
bae, Enterococcus parauberis, Enterococcus pseudoavium, Entero-
coccus raffinosus, Enterococcus saccharolyticus, and other bacteria.