Columbia Blood Agar Base, HiVeg with Blood

[所属分类:培养基配方] [发布时间:2020-5-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Columbia Blood Agar Base, HiVeg with Blood

培养基配方
Composition per liter:
Plant special peptone .....................................................23.0g
Agar.......................................................................15.0g
NaCl........................................................................5.0g
Corn starch.................................................................1.0g
Sheep blood, defibrinated.................................................50.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 950.0mL. Mix thoroughly. Gently heat until
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. For Columbia Blood Agar: Add 5% sterile defibrinated sheep
blood to sterile cool base. For Chocolate Agar: Add 10% sterile defi-
brinated sheep blood to sterile cool base. Heat to 80°C for 10 min with
constant agitation. For Selective Medium: Add desired quantity of an-
timicrobial agent to sterile base. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of fastidious microorganisms
from a variety of clinical and nonclinical specimens.