Clostridium novyi Blood Agar

[所属分类:培养基配方] [发布时间:2020-5-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium novyi Blood Agar

培养基配方
Composition per 100.0mL:
Agar .....................................................................2.0g
Glucose ..................................................................1.0g
Neopeptone................................................................1.0g
Proteolyzed liver.........................................................0.5g
Yeast extract.............................................................0.5g
Horse blood, defibrinated ..............................................10.0mL
Reducing solution.......................................................0.75mL
Salts solution...........................................................0.5mL
pH 7.6–7.8 at 25°C
Salts Solution:
Composition per 100.0mL:
MgSO4 ·7H2O................................................................................4.0g
MnSO4 ·4H2O................................................................................0.2g
HCl..............................................................................................0.05g
FeCl3 ...........................................................................................0.04g
Preparation of Salts Solution: Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly.
Reducing Solution:
Composition per 10.0mL:
L -Cysteine·HCl·H2O ...................................................................0.12g
Dithiothreitol...............................................................................0.12g
Glutamine....................................................................................0.06g
Preparation of Reducing Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Adjust
pH to 7.6–7.8. Filter sterilize.
Preparation of Medium: Add agar to distilled/deionized water and
bring volume to 50.0mL. Mix thoroughly. Gently heat and bring to
boiling. In another flask, add neopeptone, yeast extract, liver extract,
and salts solution to distilled/deionized water and bring volume to
50.0mL. Mix thoroughly. Gently heat until dissolved. Combine the two
solutions. Distribute into screw-capped bottles in 18.0mL volumes.
Autoclave for 10 min at 10 psi pressure–115°C. Cool to 45°–50°C. Me-
dium may be stored at 4°C at this point. Immediately prior to inocula-
tion, aseptically add 2.0mL of horse blood and 0.15mL of sterile
reducing solution to each tube of melted agar at 50°C. Mix thoroughly.
Pour the contents of each tube into a sterile Petri dish.
Use: For the cultivation of Clostridium novyi.