Clostridium noterae Medium

[所属分类:培养基配方] [发布时间:2020-5-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clostridium noterae Medium

培养基配方
Composition per liter:
Yeast extract..........................................................2.0g
NH4Cl .................................................................1.0g
NaCl..................................................................0.45g
K2HPO4 ·3H2O .........................................................0.4g
L -Cysteine·HCl·H2O.................................................0.15g
Na2CO3 solution .....................................................30.0mL
Trace metals solution................................................10.0mL
Na2S·9H2O solution..................................................10.0mL
pH 7.9 ± 0.1 at 25°C
Na2CO3 Solution:
Composition per 50.0mL:
Na2CO3 ................................................................5.0g
Preparation of Na2CO3 Solution : Add Na2CO3 to distilled/deion-
ized water and bring volume to 50.0mL. Autoclave for 15 min at 15 psi
pressure–121°C. Use freshly prepared solution.
Trace Metals Solution:
Composition per liter:
Na2 EDTA·2H2O..........................................................0.5g
CoCl2 ·6H2O ..........................................................0.15g
FeSO4 ·7H2O...........................................................0.1g
MnCl2 ·4H2O...........................................................0.1g
ZnCl2 .................................................................0.1g
AlCl3 ·6H2O..........................................................0.04g
CuCl2 ·2H2O .........................................................0.02g
NiSO4 ·6H2O..........................................................0.02g
H2SeO3 ...............................................................0.01g
H3BO3 ................................................................0.01g
Na2MoO4 ·2H2O .......................................................0.01g
Preparation of Trace Metals Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Na2S·9H2O Solution:
Composition per 10.0mL:
Na2S·9H2O...........................................................0.15g
Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Autoclave for 15
min at 15 psi pressure–121°C. Use freshly prepared solution.
Preparation of Medium: Add components, except Na2CO3 solu-
tion and Na2S·9H2O solution, to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Adjust pH to 7.0 with 10M NaOH.
Gently heat to boiling. Distribute under O2 -free 100% N2 gas into tubes
in 5.0mL volumes. Cap with rubber stoppers. Autoclave for 15 min at
15 psi pressure–121°C. Prior to inoculation, add to each tube 0.15mL
of Na2CO3 solution and 0.05mL of Na2S·9H2O solution. Incubate un-
der 80% H2 + 20% CO2 to provide conditions for H2  fixation.
Use:  For the cultivation and maintenance of Clostridium noterae.