Brilliant Green Bile Agar

[所属分类:培养基配方] [发布时间:2020-1-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliant Green Bile Agar

培养基配方
Composition per liter:
Noble agar................................................10.15g
Pancreatic digest of gelatin...............................8.25g
Lactose.....................................................1.9g
Na2SO3 ...................................................0.205g
Basic Fuchsin.............................................0.078g
Erioglaucine..............................................0.065g
FeCl3 ...................................................0.0295g
KH2PO4 ...................................................0.015g
Oxgall, dehydrated........................................2.95mg
Brilliant Green ..........................................0.03mg
pH 6.9 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Caution:  Basic Fuchsin is a potential carcinogen and care must be
taken to avoid inhalation of the powdered dye and contamination of the
skin.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to1.0L. For plating 10.0mL samples, prepare
the medium double strength. Mix thoroughly. Gently heat and bring to
boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Pour into sterile Petri dishes. Care should be taken to
avoid exposure of the prepared medium to light.
Use: For the detection and enumeration of coliform bacteria in mate-
rials of sanitary importance such as water, sewage, and foods. Escher-
ichia coli appears as dark red colonies with a pink halo. Enterobacter
species appear as pink colonies.