Brilliant Green Agar with Sulfadiazine

[所属分类:培养基配方] [发布时间:2020-1-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliant Green Agar with Sulfadiazine

培养基配方

Composition per liter:
Agar ..........................................................20.0g
Lactose........................................................10.0g
Sucrose........................................................10.0g
Pancreatic digest of casein.....................................5.0g
Peptic digest of animal tissue..................................5.0g
NaCl............................................................5.0g
Yeast extract...................................................3.0g
Phenol Red.....................................................0.08g
Sulfadiazine ..................................................0.08g
Brilliant Green .............................................0.0125g
pH 6.9 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes.
Use: For the selective detection of Salmonella in foods, especially from
egg products. Salmonella other than Salmonella typhi appear as red/pink
colonies surrounded by a zone of red in the agar indicating nonlactose/
sucrose fermentation. Proteus or Pseudomonas species may appear as
small red colonies. Lactose- or sucrose-fermenting bacteria appear as yel-
low-green colonies surrounded by a zone of yellow-green in the agar.