Brackish Acetate

[所属分类:培养基配方] [发布时间:2020-1-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brackish Acetate

培养基配方
Composition per liter:
Sodium acetate...............................................1.0g
KNO3 ........................................................1.0g
NaH2PO4 ·2H2O..............................................0.05g
Artificial seawater.......................................250.0mL
Modified Hutner’s basal salts.............................20.0mL
Vitamin solution...........................................10.0mL
pH 7.2 ± 0.2 at 25°C
Artificial Seawater:
Composition per liter:
NaCl........................................................23.5g
MgCl2 .......................................................5.0g
Na2SO4 ......................................................3.9g
CaCl2 .......................................................1.1g
KCl..........................................................0.66g
NaHCO3 ......................................................0.19g
KBr ..........................................................0.1g
H3BO3 ......................................................0.026g
SrCl2 ......................................................0.024g
NaF .........................................................3.0mg
Preparation of Artificial Seawater:  Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly.
Modified Hutner’s Basal Salts:
Composition per liter:
MgSO4· 7H2O....................................................29.7g
Nitrilotriacetic acid...........................................10.0g
CaCl2 ·2H2O ...................................................3.34g
FeSO4 ·7H2O....................................................0.1g
(NH4)2 MoO4 ...................................................9.25mg
Metals “44”..................................................50.0mL
Preparation of Modified Hutner’s Basal Salts:  Dissolve the
nitrilotriacetic acid first and neutralize the solution with KOH. Add the
other components and adjust the pH to 7.2 with KOH or H 2 SO 4 . There
may be a slight precipitate. Store at 5°C.
Metals “44”:
Composition per 100.0mL:
ZnSO4 ·7H2O..................................................1.1g
FeSO4 ·7H2O..................................................0.5g
EDTA........................................................0.25g
MnSO4 ·7H2O................................................0.154g
CuSO4 ·5H2O.................................................0.04g
Co(NO3)2 ·6H2O ............................................0.025g
Na2B4O7 ·10H2O..............................................0.018g
Preparation of Metals “44”:  Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Add aseptically to sterile basal
medium.
Vitamin Solution:
Composition per liter:
Thiamine·HCl ...............................................5.0mg
D- Calcium pantothenate......................................5.0mg
Riboflavin ..................................................5.0mg
Biotin ......................................................2.0mg
Folic acid...................................................2.0mg
Vitamin B 12 ................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize and add aseptically to sterile basal medium.
Preparation of Medium: Add a few drops of H 2 SO 4 to the distilled
water to retard precipitation of the metal salts. Add components, except
for Metals “44” and vitamin solutions to 250.0mL of artificial seawater
and 720.0mL of distilled/deionized water. Adjust pH to 7.2. Distribute
into tubes or flasks. Sterilize by autoclaving for 15 min at 15 psi pres-
sure–121°C. Aseptically add Metals “44” and vitamin solution. Mix
thoroughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation of Filomicrobium fusiforme.