B.Q.Vaccine Medium with Glucose (Thioglycollate Broth with Liver Extract)

[所属分类:培养基配方] [发布时间:2020-1-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

B.Q.Vaccine Medium with Glucose
(Thioglycollate Broth with Liver Extract)

培养基配方
Composition per liter:
Liver tissues............................................250.0g
Muscle tissues...........................................250.0g
Peptic digest of animal tissue............................10.0g
NaCl.......................................................5.0g
K2HPO4 ....................................................4.0g
Na-thioglycollate .........................................1.0g
Glucose solution ........................................50.0mL
pH 8.2 ± 0.2 at 25°C
Source: This medium without glucose solution is available as a pre-
mixed powder from HiMedia.
Glucose Solution:
Composition per 100.0mL:
Glucose..................................................20.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 950.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptical-
ly add 50.0mL of sterile glucose solution. Mix thoroughly. Aseptically
distribute into sterile tubes or flasks.
Use: For the cultivation of anaerobic organisms on a large scale.