BPHD Medium (DSMZ Medium 738)

[所属分类:培养基配方] [发布时间:2020-1-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BPHD Medium
(DSMZ Medium 738)

培养基配方
Composition per liter:
NH4Cl ....................................................1.0g
Na2HPO4 ·2H2O...........................................0.42g
Na2H2PO4 ·H2O ..........................................0.18g
MgCl2 ·6H2O..............................................0.1g
CaCl2 ·2H2O..............................................0.1g
KCl.......................................................0.1g
FeSO4 ·7H2O.............................................0.04g
Phenol solution ........................................20.0mL
Vitamin solution........................................10.0mL
Trace elements solution ................................10.0mL
pH 6.5 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
MgSO4 ·7H2O.............................................3.0g
Nitrilotriacetic acid....................................1.5g
NaCl.....................................................1.0g
MnSO4 ·2H2O.............................................0.5g
CoSO4 ·7H2O............................................0.18g
ZnSO4 ·7H2O............................................0.18g
CaCl2 ·2H2O.............................................0.1g
FeSO4 ·7H2O.............................................0.1g
NiCl2 ·6H2O...........................................0.025g
KAl(SO4)2 ·12H2O.......................................0.02g
H3BO3 ..................................................0.01g
Na2MoO4 ·4H2O..........................................0.01g
CuSO4 ·5H2O............................................0.01g
Na2SeO3 ·5H2O..........................................0.3mg
Preparation of Trace Elements Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Pyridoxine-HCl............................................10.0mg
Thiamine-HCl·2H2O.........................................5.0mg
Riboflavin.................................................5.0mg
Nicotinic acid.............................................5.0mg
D -Ca-pantothenate ........................................5.0mg
p-Aminobenzoic acid........................................5.0mg
Lipoic acid................................................5.0mg
Biotin.....................................................2.0mg
Folic acid ................................................2.0mg
Vitamin B 12 ..............................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Phenol Solution:
Composition per liter:
Phenol......................................................4.7g
Preparation of Phenol Solution:  Add phenol to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except phenol solution
and vitamin solution, to distilled/deionized water and bring volume to
970.0mL. Mix thoroughly. Adjust pH to 6.5. Autoclave for 15 min at 15
psi pressure–121°C. Aseptically add 20.0mL phenol solution and
10.0mL vitamin solution. Mix thoroughly. Aseptically distribute into
sterile tubes or bottles.
Use:  For the cultivation of Bacillus sp.