Bovine Serum Albumin Tween™ 80 Soft Agar (BSA Tween™ 80 Soft Agar) (Semisolid BSA Tween™ 80 Medium)

[所属分类:培养基配方] [发布时间:2020-1-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bovine Serum Albumin Tween™ 80 Soft Agar
(BSA Tween™ 80 Soft Agar)
(Semisolid BSA Tween™ 80 Medium)

培养基配方
Composition per liter:
Basal medium ............................................900.0mL
Albumin supplement.......................................100.0mL
Basal Medium:
Composition per liter:
Agar ......................................................2.0g
Na2HPO4 ...................................................1.0g
NaCl.......................................................1.0g
KH2PO4 ....................................................0.3g
Glycerol (10% solution)...................................1.0mL
NH4Cl (25% solution)......................................1.0mL
Sodium pyruvate (10% solution)............................1.0mL
Thiamine (0.5% solution)..................................1.0mL
Preparation of Basal Medium: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to
7.4. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Albumin Supplement:
Composition per 100.0mL:
Bovine albumin.......................................10.0g
Tween ™ 80 (10% solution) ..........................12.5mL
FeSO4 (0.5% solution)...............................10.0mL
CaCl2 –MgCl2 solution...............................1.0mL
Cyanocobalamin (0.02% solution) .....................1.0mL
ZnSO4 (0.4% solution)................................1.0mL
Preparation of Albumin Supplement: Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Adjust pH to 7.4. Filter sterilize.
CaCl2 –MgCl2 Solution:
Composition per 100.0mL:
CaCl2 ·2H2O..............................................1.5g
MgCl2 ·6H2O..............................................1.5g
Preparation of CaCl2 –MgCl2 Solution: Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: To 900.0mL of cooled, sterile basal me-
dium, aseptically add 100.0mL of sterile albumin supplement. Mix
thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Leptospira species.