BL Agar (Glucose Blood Liver Agar)

[所属分类:培养基配方] [发布时间:2019-12-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BL Agar
(Glucose Blood Liver Agar)

培养基配方
Composition per liter:
Agar .......................................................15.0g
Glucose ....................................................10.0g
Proteose peptone No.3.......................................10.0g
Pancreatic digest of casein..................................5.0g
Yeast extract................................................5.0g
Meat extract ................................................3.0g
Phytone™ ....................................................3.0g
Tween™ 80....................................................1.0g
Soluble starch...............................................0.5g
Liver extract.............................................150.0mL
Horse blood................................................50.0mL
L -Cysteine·HCl solution..................................10.0mL
Solution A.................................................10.0mL
Solution B..................................................5.0mL
pH 7.2 ± 0.2 at 25°C
Liver Extract:
Composition per 170.0mL:
Liver powder................................................10.0g
Preparation of Liver Extract:  Add 10.0g of liver powder to
170mL of distilled/deionized water. Gently heat to 60°C. Maintain at
50°–60°C for 1 hr. Gently bring to boiling. Boil for 5 min. Adjust pH
to 7.2. Filter through Whatman #2 filter paper.
L -Cysteine·HCl Solution:
Composition per 10.0mL:
L -Cysteine·HCl............................................0.5g
Preparation of  L -Cysteine·HCl Solution:  Dissolve 0.5g of  L -
cysteine·HCl in distilled/deionized water and bring volume to 10.0mL.
Mix thoroughly. Filter sterilize. Warm to 50°C.
Solution A:
Composition per 100.0mL:
K2HPO4 ....................................................10.0g
KH2PO4 ....................................................10.0g
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°–55°C.
Solution B:
Composition per 100.0mL:
MgSO4 ·7H2O.................................................4.0g
NaCl.........................................................0.2g
FeSO4 ·7H2O.................................................0.2g
MnSO4 ·H2O..................................................0.2g
Preparation of Solution B:  Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°–55°C.
Preparation of Medium: Add components, except liver extract,
horse blood,  L -cysteine·HCl solution, solution A, and solution B, to
distilled/deionized water and bring volume to 775.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°–55°C. Aseptically add 150.0mL of sterile
liver extract, 50.0mL of sterile horse blood, 10.0mL of sterile  L -
cysteine·HCl solution, 10.0 mL of sterile solution A, and 5.0mL of ster-
ile solution B. Mix thoroughly. Pour into sterile Petri dishes or distrib-
ute into sterile tubes.
Use: For the cultivation and maintenance of Atopobium minutum,
Bacteroides distasonis, Bacteroides ovatus, Bacteroides thetaiotaomi-
cron, Bacteroides uniformis, Bacteroides vulgatus, numerous Bifido-
bacterium species, Campylobacter divergens, Carnobacterium pisci-
cola, numerous Clostridium species, numerous Lactobacillus species,
Lactococcus lactis, Leuconostoc lactis, Leuconostoc mesenteroides,
and Propionibacterium thoenii.