Bismuth Sulfite Broth (m-Bismuth Sulfite Broth)

[所属分类:培养基配方] [发布时间:2019-12-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bismuth Sulfite Broth
(m-Bismuth Sulfite Broth)

培养基配方
Composition per liter:
Bi2(SO3)3 .....................................................16.0g
Pancreatic digest of casein....................................10.0g
Peptic digest of animal tissue ................................10.0g
Beef extract...................................................10.0g
Glucose........................................................10.0g
Na2HPO4 ........................................................8.0g
FeSO4 ·7H2O....................................................0.6g
pH 7.7 ± 0.2 at 25°C
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly and heat with frequent
agitation until boiling. Boil for 1 min. Do not autoclave. Cool to 45°–
50°C. Mix to disperse the precipitate and aseptically distribute into
sterile tubes or flasks. Use 2.0–2.2mL of medium for each membrane
filter.
Use: For the selective isolation of Salmonella typhi and other enteric
bacilli and for the detection of Salmonella by the membrane filter
method.