Bile Broth Base, HiVeg with Streptokinase

[所属分类:培养基配方] [发布时间:2019-12-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Bile Broth Base, HiVeg with Streptokinase

培养基配方
Composition per liter:
Plant peptone ..............................................20.0g
NaCl.........................................................5.0g
Synthetic detergent No. V....................................5.0g
Streptokinase solution......................................1.0mL
pH 7.1 ± 0.2 at 25°C
Source: This medium, without streptokinase solution, is available as
a premixed powder from HiMedia.
Streptokinase Solution:
Composition per 1.0mL:
Streptokinase.......................................100,000 units
Streptokinase Solution: Add streptokinase to distilled/deionized
water and bring volume to 1.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Heat gently and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 40°C. Aseptically add 1.0mL of streptoki-
nase solution. Mix thoroughly. If desired carbohydrate may also be
added to this medium prior to sterilization.
Use: For the culture of blood clots from patients with suspected enteric
fever.