BiGGY Agar (Bismuth Sulfite Glucose Glycerin Yeast Extract Agar) (Nickerson Medium)

[所属分类:培养基配方] [发布时间:2019-12-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

BiGGY Agar
(Bismuth Sulfite Glucose
Glycerin Yeast Extract Agar)
(Nickerson Medium)

培养基配方
Composition per liter:
Agar..........................................................16.0g
Glucose.......................................................10.0g
Glycine.......................................................10.0g
Bismuth ammonium citrate.......................................5.0g
Na2SO3 ........................................................3.0g
Yeast extract..................................................1.0g
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid
Unipath and BD Diagnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly and heat with frequent
agitation until boiling. Distribute into tubes or flasks. Do not autoclave.
Cool to approximately 45°–50°C. If desired, add 2mg/L of neomycin
sulfate. Swirl to disperse the insoluble material and pour into sterile Pe-
tri dishes.
Use: For the detection, isolation, and presumptive identification of
Candida species. Addition of neomycin helps inhibit bacterial species.
Candida albicans appears as brown to black colonies with no pigment
diffusion and no sheen. Candida tropicalis appears as dark brown col-
onies with black centers, black pigment diffusion, and a sheen. Can-
dida krusei appears as shiny, wrinkled, brown to black colonies with
yellow pigment diffusion. Candida pseudotropicalis appears as flat,
shiny red to brown colonies with no pigment diffusion. Candida
parakrusei appears as flat, shiny, wrinkled, dark reddish-brown colo-
nies with light reddish-brown peripheries and a yellow fringe. Candida
stellatoidea appears as flat dark brown colonies with a light fringe.