BHI/2 Medium

[所属分类:培养基配方] [发布时间:2019-12-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

BHI/2 Medium

培养基配方
Composition per liter:
Pancreatic digest of gelatin...............................14.5g
Casein hydrolysate.........................................10.0g
Glucose.....................................................8.0g
Brain heart, solids from infusion...........................6.0g
Peptic digest of animal tissue .............................6.0g
NaCl........................................................5.0g
Yeast extract...............................................5.0g
Na2HPO4 ....................................................2.5g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Actinomyces georgiae,
Actinomyces gerencseriae, Actinomyces naeslundii, Actinomyces
odontolyticus, and Actinomyces viscosus.