BG 11 Marine Agar (Medium BG 11 for Marine Cyanobacteria)

[所属分类:培养基配方] [发布时间:2019-12-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

BG 11 Marine Agar
(Medium BG 11 for Marine Cyanobacteria)
Composition per liter:
Agar ........................................................10.0g
NaCl.........................................................10.0g
NaNO3 ........................................................1.5g
MgSO4 ·7H2O................................................0.075g
K2HPO4 ......................................................0.04g
CaCl2 ·2H2O................................................0.036g
Na2CO3 ......................................................0.02g
Citric acid..................................................6.0mg
Ferric ammonium citrate......................................6.0mg
EDTA disodium salt...........................................1.0mg
Vitamin B 12 solution......................................100.0mL
Trace metal mix A5...........................................1.0mL
pH 7.1 ± 0.2 at 25°C
Trace Metal Mix A5:
Composition per liter:
H3BO3 .......................................................2.86g
MnCl2 ·4H2O.................................................1.81g
Na2MoO4 ·2H2O ..............................................0.39g
ZnSO4 ·7H2O................................................0.222g
CuSO4 ·5H2O................................................0.079g
Co(NO3)2 ·6H2O ............................................0.049g
Preparation of Trace Metal Mix A5: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin B 12  Solution:
Composition per 100.0mL:
Vitamin B 12 ..............................................1.0μg
Preparation of Vitamin B 12 Solution: Add vitamin B 12 to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except vitamin B 12 so-
lution, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Heat gently to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Aseptically add 100.0mL of sterile vitamin B 12 solution.
Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Synechococcus species.
For the isolation of cyanobacteria from freshwater habitats.