BG 11 Agar (Medium BG 11 for Cyanobacteria)

[所属分类:培养基配方] [发布时间:2019-12-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

BG 11 Agar
(Medium BG 11 for Cyanobacteria)

培养基配方
Composition per liter:
Agar..........................................................10.0g
NaNO3 .........................................................1.5g
MgSO4 ·7H2O.................................................0.075g
K2HPO4 .......................................................0.04g
CaCl2 ·2H2O ................................................0.036g
Na2CO3 .......................................................0.02g
Citric acid...................................................6.0mg
Ferric ammonium citrate.......................................6.0mg
Disodium EDTA.................................................1.0mg
Trace metal mix A5 ...........................................1.0mL
pH 7.1 ± 0.2 at 25°C
Trace Metal Mix A5:
Composition per liter:
H3BO3 ........................................................2.86g
MnCl2 ·4H2O .................................................1.81g
Na2MoO4 ·2H2O................................................0.39g
ZnSO4 ·7H2O.................................................0.222g
CuSO4 ·5H2O.................................................0.079g
Co(NO3)2 ·6H2O..............................................0.049g
Preparation of Trace Metal Mix A5: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Heat gently to boil-
ing. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pres-
sure–121°C. For solid medium, pour into sterile Petri dishes or leave in
tubes.
Use:  For the cultivation and maintenance of a variety of cyanobacteria,
including Anabaena species, Calothrix species, Chaemisiphon species,
Chorogloeopsis species, Chroococcidiopsis species, Cylindrospermum
species, Dermocarpa species, Fischerella species, Gloebacter species,
Gloeocapsa species, Gloeothece species, Nostoc species, Oscillatoria spe-
cies, Phormidium species, Pleurocapsa species, Pseudanabaena species,
Scytonema species, Spirulina species, Synechococcus species, and Syn-
echocystis species.