Beggiatoa Broth

[所属分类:培养基配方] [发布时间:2019-12-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Beggiatoa Broth

培养基配方
Composition per 1010.0mL:
Sodium acetate...........................................0.5g
Pancreatic digest of gelatin............................0.31g
Beef extract............................................0.19g
NH4Cl .................................................0.45mg
MgSO4 ·7H2O............................................0.2mg
K2HPO4 .................................................0.1mg
CaSO4  (saturated solution)............................20.0mL
Catalase solution......................................10.0mL
Trace elements solution ................................5.0mL
pH 7.4 ± 0.2 at 25°C
Catalase Solution:
Composition per 10.0mL:
Catalase........................................15,000–35,000U
Preparation of Catalase Solution:  Add catalase to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Trace Elements Solution:
Composition per liter:
FeSO4 ·7H2O...............................................0.7g
EDTA.......................................................0.2g
ZnSO4 ·7H2O..............................................0.01g
MnSO4 ·4H2O.............................................0.002g
H3BO3 ...................................................10.0mg
CO(NO3)2 ................................................1.0mg
Na2MoO4 ·2H2O...........................................1.0mg
CuSO4 ·5H2O............................................5.0μg
Preparation of Trace Elements Solution:  Add FeSO 4 ·7H2O to
10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized wa-
ter and bring volume to 1.0L. Add remaining components. Mix thor-
oughly.
Preparation of Medium: Add components, except catalase solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Adjust pH to 7.4. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 10.0mL of sterile catalase solution (freshly pre-
pared). Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation of Beggiatoa alba.