Beggiatoa and Thiothrix Medium

[所属分类:培养基配方] [发布时间:2019-12-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Beggiatoa and Thiothrix Medium

培养基配方
Composition per liter:
CaSO4 ·2H2O (saturated solution) ......................20.0mL
NH4Cl (4% solution)......................................5.0mL
Trace elements...........................................5.0mL
K2HPO4 (1% solution).....................................1.0mL
MgSO4 ·7H2O (1% solution) ..............................1.0mL
Trace Elements:
Composition per liter:
EDTA solution...........................................20.0mL
Co(NO3)2 (0.01% solution)...............................10.0mL
CuSO4 ·5H2O (0.00005% solution)........................10.0mL
H3BO3 (0.1% solution) ..................................10.0mL
MnSO4 ·4H2O (0.02% solution) ..........................10.0mL
Na2MoO4 ·2H2O (0.01% solution) ........................10.0mL
ZnSO4 ·7H2O (0.1% solution) ...........................10.0mL
Preparation of Trace Elements:  Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
EDTA Solution:
Composition per 100.0mL:
FeSO4 ..................................................7.0g
EDTA....................................................2.0g
HCl, concentrated .....................................1.0mL
Preparation of EDTA Solution: Add EDTA and FeSO 4 to con-
centrated HCl. Mix thoroughly. Carefully add to distilled/deionized
water and bring volume to 100.0mL.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Beggiatoa species and myxotrophic Thio-
thrix species.