Beef Liver Medium for Anaerobes

[所属分类:培养基配方] [发布时间:2019-12-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Beef Liver Medium for Anaerobes

培养基配方
Composition per liter:
Beef liver, minced............................................500.0g
Peptone .......................................................10.0g
K2HPO4 .........................................................1.0g
pH 8.0 ± 0.2 at 25°C
Preparation of Medium:  Add beef liver to 1.0L of tap water. Soak
for 12–24 hr at 4°C. Skim fat off top. Autoclave for 10 min at 15 psi pres-
sure–121°C. Filter through cheesecloth. Save meat. To filtrate, add pep-
tone and K2HPO4 . Adjust pH to 8.0. Filter through paper. Add tap water
and bring volume to 1.0L. Add a small amount of CaCO3 to a flask or test
tube. Add 0.5 inch of reserved liver. Cover meat with 2 inches of broth.
Cap tubes and autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of a variety of Clostridium
species.