Beef Infusion Broth

[所属分类:培养基配方] [发布时间:2019-12-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Beef Infusion Broth

培养基配方
Composition per liter:
Ground beef, defatted ....................................453.6g
Peptone....................................................10.0g
NaCl.......................................................5.0g
pH 7.6 ± 0.2 at 25°C
Preparation of Medium: Add ground beef to 1.0L of distilled/deion-
ized water. Let stand overnight at 4°C. Gently heat and bring to 80°–90°C
for 60 min. Let stand for 2 hr. Filter through muslin. To filtrate add peptone
and salt. Mix thoroughly. Adjust pH to 7.6 with 4% NaOH. Filter through
Whatman #1 filter paper. Bring volume of filtrate to 1.0L. Add agar. Gen-
tly heat and bring to boiling. Distribute into tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C.
Use: For the cultivation of a variety of microorganisms.