BCYEα without L -Cysteine (Buffered Charcoal Yeast Extract Agar without L -Cysteine)

[所属分类:培养基配方] [发布时间:2019-12-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BCYEα without  L -Cysteine
(Buffered Charcoal Yeast Extract Agar
without  L -Cysteine)

培养基配方
Composition per liter:
Agar ......................................................15.0g
Yeast extract..............................................10.0g
ACES buffer (2-[(2-amino-2-oxoethyl)-
amino]-ethane sulfonic acid)...............................10.0g
Charcoal, activated.........................................2.0g
α-Ketoglutarate............................................1.0g
Fe4(P2O7)3 ·9H2O solution................................10.0mL
pH 6.9 ± 0.2 at 25°C
Fe4(P2O7)3 ·9H2O Solution:
Composition per 10.0mL:
Fe4 (P2O7)3 ·9H2O........................................0.25g
Preparation of Fe4(P2O7)3 ·9H2O Solution: Add Fe4(P2O7)3 ·9H2O
to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium:  Add components, except Fe 4 (P 2 O 7 ) 3 ·9H 2 O
solution, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Adjust medium to pH 6.9 with 1N KOH. Heat gently and bring
to boiling for 1 min. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°–55°C. Aseptically add 10.0mL of sterile Fe 4 (P 2 O 7 ) 3 ·9H 2 O solu-
tion. Mix thoroughly. Pour into sterile Petri dishes with constant agitation
to keep charcoal in suspension.
Use: For the isolation, cultivation, and maintenance of Legionella
pneumophila and other Legionella species from environmental and
clinical specimens.