BCYE Differential Agar (Buffered Charcoal Yeast Extract Differential Agar)

[所属分类:培养基配方] [发布时间:2019-12-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BCYE Differential Agar
(Buffered Charcoal Yeast Extract Differential Agar)

培养基配方
Composition per liter:
Agar..........................................................15.0g
Yeast extract.................................................10.0g
ACES buffer (2-[(2-amino-2-oxoethyl)-
amino]-ethane sulfonic acid)..................................10.0g
Charcoal, activated ...........................................2.0g
α-Ketoglutarate...............................................1.0g
L- Cysteine·HCl·H2O .........................................0.4g
Fe4(P2O7)3 ·9H2O.............................................0.25g
Bromcresol Purple ............................................0.01g
Bromthymol Blue ..............................................0.01g
pH 6.9 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation  of  Medium:  Add  components,  except  L -
cysteine·HCl·H2O, to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Adjust medium to pH 6.9 with 1N KOH. Heat
gently and bring to boiling for 1 min. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°–55°C. Add 4.0mL of a 10% solution of
L- cysteine·HCl·H 2 O that has been filter sterilized. Mix thoroughly.
Pour into sterile Petri dishes with constant agitation to keep charcoal in
suspension.
Use: For the isolation, cultivation, and maintenance of Legionella
pneumophila and other Legionella species from environmental and
clinical specimens. For the presumptive differential identification of
Legionella species based on colony color and morphology. Legionella
pneumophila appears as light blue/green colonies. Legionella micdadei
appears as blue/gray or dark blue colonies.