BCYE Agar with Cysteine (BCYE Alpha Base) (Buffered Charcoal Yeast Extract Agar)

[所属分类:培养基配方] [发布时间:2019-12-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BCYE Agar with Cysteine
(BCYE Alpha Base）

培养基配方
(Buffered Charcoal Yeast Extract Agar)
Composition per liter:
Agar........................................................15.0g
Yeast extract...............................................10.0g
ACES buffer (2-[(2-amino-2-oxoethyl)-
amino]-ethane sulfonic acid)................................10.0g
Charcoal, activated ........................................2.0g
α-Ketoglutarate............................................1.0g
L- Cysteine·HCl·H2O ......................................0.4g
Fe4(P2O7)3 ·9H2O.........................................0.25g
L -Cysteine solution.......................................4.0mL
pH 6.9 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
L -Cysteine Solution:
Composition per 10.0mL:
L- cysteine·HCl·H2O .......................................0.4g
Preparation of  L -Cysteine Solution: Add  L- cysteine·HCl·H2O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium:  Add components, except  L- cysteine solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Adjust medium to pH 6.9 with 1N KOH. Heat gently and bring
to boil for 1 min. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°–55°C. Aseptically add 4.0mL of  L- cysteine solution. Mix thor-
oughly. Pour into sterile Petri dishes with constant agitation to keep
charcoal in suspension.
Use: For the isolation, cultivation, and maintenance of Legionella
pneumophila and other Legionella species from environmental and
clinical specimens.