Baird-Parker Agar

[所属分类:培养基配方] [发布时间:2019-12-4] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Baird-Parker Agar

培养基配方
Composition per liter:
Agar.............................................................17.0g
Glycine..........................................................12.0g
Sodium pyruvate..................................................10.0g
Pancreatic digest of casein......................................10.0g
Beef extract......................................................5.0g
LiCl..............................................................5.0g
Yeast extract.....................................................1.0g
Sulfamethazine solution........................................10.0mL
pH 7.0 ± 0.2 at 25°C
Sulfamethazine Solution:
Composition per 10.0mL:
Sulfamethazine ..................................................0.05g
Preparation of Sulfamethazine Solution: Add sulfamethazine
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except sulfamethazine
solution, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile sulfamet-
hazine solution. Mix thoroughly. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: Used as a base for the preparation of egg-tellurite-glycine-pyru-
vate agar for the selective isolation and enumeration of coagulase-pos-
itive staphylococci from food, skin, soil, air, and other materials.