ASLA Agar

[所属分类:培养基配方] [发布时间:2019-11-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

ASLA Agar
Composition per liter:
Sodium lactate ............................................................................20.0g
Davis agar...................................................................................10.0g
(NH4)2SO4 ....................................................................................3.0g
Na2HPO4 .......................................................................................1.2g
L- Cysteine·HCl..............................................................................0.5g
MgSO4 ·7H2O................................................................................0.2g
MnSO4 ·4H2O..............................................................................0.05g
FeSO4 ·7H2O................................................................................0.04g
Vitamin solution.......................................................................10.0mL
pH 6.5 ± 0.2 at 25°C
Vitamin Solution:
Composition per liter:
Biotin............................................................................................0.1g
Calcium pantothenate ...................................................................0.1g
p-Aminobenzoic acid....................................................................0.1g
Thiamine.......................................................................................0.1g
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize. Store solution at −20°C.
Preparation of Medium: Add components, except vitamin solution,
to distilled/deionized water and bring volume to 990.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile vitamin
solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the selective isolation and cultivation of most Propionibac-
terium species from foods.