Ashdown’s Medium

[所属分类:培养基配方] [发布时间:2019-11-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ashdown’s Medium
Composition per liter:
Pancreatic digest of casein..........................................................14.5g
Agar............................................................................................14.0g
Papaic digest of soybean meal......................................................5.0g
NaCl..............................................................................................5.0g
Neutral Red..............................................................................50.0mg
Crystal Violet.............................................................................5.0mg
Gentamicin.................................................................................4.0mg
Glycerol ...................................................................................40.0mL
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Autoclave for 15 min at 15 psi pres-
sure–121°C. Pour into sterile Petri dishes or leave iin tubes.
Use: For the isolation and cultivation of Burkholderia pseudomallei
from clinical specimens.