Arcobacter Medium

[所属分类:培养基配方] [发布时间:2019-11-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Arcobacter Medium
Composition per liter:
Agar............................................................................................15.0g
Special peptone...........................................................................10.0g
Beef extract...................................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................5.0g
Sodium glutamate.........................................................................2.0g
Sodium succinate..........................................................................2.0g
MgCl2 ·H2O ...................................................................................1.0g
Horse blood, defibrinated ........................................................50.0mL
pH 7.0 ± 0.2 at 25°C
Source:  Special peptone is available from Oxoid Unipath.
Preparation of Medium: Add components, except horse blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Adjust pH to 7.0. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add
50.0mL of sterile horse blood. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Arcobacter species.