Arcobacter Broth Base with Selective Supplement

[所属分类:培养基配方] [发布时间:2019-11-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Arcobacter Broth Base with Selective Supplement
Composition per liter:
Peptone........................................................................................18.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................1.0g
Selective supplement solution .................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Cefoperazone...........................................................................16.0mg
Amphotericin B .........................................................................5.0mg
Preparation of Selective Supplement Solution:  Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add selective supplement solution.
Mix thoroughly. Pour into Petri dishes or aseptically distribute into
sterile tubes.
Use: For the enrichment and cultivation of Arcobacter spp.