Archaeoglobus Medium

[所属分类:培养基配方] [发布时间:2019-11-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Archaeoglobus Medium
Composition per liter:
NaCl............................................................................................18.0g
NaHCO3 ........................................................................................5.0g
MgCl2·6H2O .................................................................................4.0g
MgSO4·7H2O..............................................................................3.45g
Sodium  L- lactate ...........................................................................1.5g
Yeast extract..................................................................................0.5g
KCl..............................................................................................0.34g
NH4Cl .........................................................................................0.25g
CaCl2·2H2O ................................................................................0.14g
K2HPO4 .......................................................................................0.14g
Fe(NH4)2(SO4)2·7H2O...............................................................2.0mg
Resazurin ...................................................................................1.0mg
Na2S·9H2O solution.................................................................25.0mL
Trace elements solution...........................................................10.0mL
pH 6.9 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
MgSO4·7H2O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO4 ·2H2O................................................................................0.5g
CoSO4 ·7H2O...............................................................................0.18g
ZnSO4 ·7H2O...............................................................................0.18g
FeSO4 ·7H2O..................................................................................0.1g
CaCl2 ·2H2O ..................................................................................0.1g
NiCl2 ·6H2O...............................................................................0.025g
KAl(SO4)2·l2H2O .......................................................................0.02g
CuSO4·5H2O...............................................................................0.01g
H3BO3 .........................................................................................0.01g
Na2MoO4 ·2H2O..........................................................................0.01g
Na2SeO3 ·5H2O...........................................................................0.3mg
Na2S·9H2O Solution:
Composition per 50.0mL:
Na2S·9H2O....................................................................................1.0g
Preparation of Na 2 S·9H 2 O Solution: Prepare and dispense solu-
tion anaerobically under 80% N 2 + 20% CO 2 . Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 50.0mL. Mix thoroughly.
Adjust pH to 7.0. Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Trace Elements Solution: Add nitrilotriacetic
acid to approximately 500.0mL of distilled/deionized water. Dissolve
by adding KOH and adjust pH to 6.5. Add remaining components.
Bring volume to 1.0L with additional distilled/deionized water. Adjust
pH to 7.0 with KOH.
Preparation of Medium: Add components, except NaHCO 3 and
Na 2 S·9H 2 O solution, to distilled/deionized water and bring volume to
1.0L. Mix well and heat to boiling for a few minutes. Cool rapidly to
room temperature while gassing with 80% N 2 + 20% CO 2 . Add
NaHCO3 and adjust pH to 6.9. Distribute anaerobically under 80% N 2
+ 20% CO 2 and pressurize sealed containers up to 2 bar pressure. Au-
toclave for 15 min at 15 psi pressure–121°C. Prior to inoculation of
cultures, add 0.25mL of sterile Na 2 S·9H 2 O solution to each tube con-
taining 9.75mL of sterile basal medium.
Use: For the cultivation and maintenance of Archaeoglobus fulgidus.