Aquaspirillum Autotrophic Agar

[所属分类:培养基配方] [发布时间:2019-11-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Aquaspirillum Autotrophic Agar
Composition per liter:
Noble agar...................................................................................15.0g
Na2HPO4 ·12H 2 O...........................................................................9.0g
KH2PO4 .........................................................................................1.5g
NH4Cl ...........................................................................................1.0g
MgSO4 ·7H2O................................................................................0.2g
CaCl2 ·2H2O.................................................................................0.01g
Ferric ammonium citrate............................................................5.0mg
NaHCO3 solution.....................................................................10.0mL
Trace elements solution .............................................................3.0mL
pH 7.1 ± 0.2 at 25°C
NaHCO3 Solution:
Composition per 10.0mL:
NaHCO3 ........................................................................................0.5g
Preparation of NaHCO 3 Solution: Add the NaHCO 3 to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Trace Elements Solution:
Composition per liter:
H3BO3 ......................................................................................30.0mg
CoCl2 ·6H2O.............................................................................20.0mg
ZnSO4 ·7H2O............................................................................10.0mg
MnCl2 ·4H2O ..............................................................................3.0mg
Na2MoO4·2H2O.........................................................................3.0mg
NiCl2 ·6H2O................................................................................2.0mg
CuCl2 ·2H2O...............................................................................1.0mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except NaHCO 3 solu-
tion, to double-distilled water and bring volume to 990.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add sterile NaHCO 3 so-
lution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes. For autotrophic growth, incubate under 85% H 2 + 10% CO 2
+ 5% O 2 .
Use: For the autotrophic cultivation and maintenance of Aquaspiril-
lum autotrophicum.