Aolpha Medium

[所属分类:培养基配方] [发布时间:2019-11-4] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Aolpha Medium
Composition per 1041.0mL:
NaCl..........................................................................................100.0g
Agar............................................................................................15.0g
MgSO4 ·7H20................................................................................9.5g
MgCl2 ·6H2O .................................................................................5.0g
KCl................................................................................................5.0g
Peptone .........................................................................................5.0g
Yeast extract..................................................................................1.0g
CaCl2·2H2O ..................................................................................0.2g
(NH4 )2SO4 ....................................................................................0.1g
KNO3 ............................................................................................0.1g
Metals solution.........................................................................20.0mL
Phosphate solution...................................................................20.0mL
Vitamin solution.........................................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Metals Solution:
Composition per liter:
MgSO4 ·7H2O..............................................................................29.7g
Nitrilotriacetic acid.....................................................................10.0g
CaCl2 ·2H2O ..................................................................................3.3g
FeSO4 ·7H2O.............................................................................99.0mg
Na2MoO4·2H2O.......................................................................12.7mg
Metals “44”..............................................................................50.0mL
Preparation of Metals Solution:  Solubilize nitrilotriacetic acid
with KOH. Dissolve remaining ingredients. Adjust pH to 7.2 with
KOH or H2SO4 . Autoclave for 15 min at 15 psi pressure–121°C. Add
aseptically to sterile basal medium.
Metals “44”:
Composition per 100.0mL:
ZnSO4 ·7H2O.................................................................................1.1g
FeSO4 ·7H2O..................................................................................0.5g
EDTA..........................................................................................0.25g
MnSO4 ·7H2O............................................................................0.154g
CuSO4 ·5H2O...............................................................................0.04g
Co(NO3)2·6H2O........................................................................0.025g
Na2B4O7·10H2O........................................................................0.018g
Preparation of Metals “44”:  Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Add aseptically to sterile basal
medium.
Phosphate Solution:
Composition per liter:
K2HPO4 .........................................................................................2.5g
KH2PO4 .........................................................................................2.5g
Preparation of Phosphate Solution:  Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure–121°C. Add aseptically to sterile
basal medium.
Vitamin Solution:
Composition per liter:
Pyridoxine·HCl........................................................................10.0mg
Calcium pantothenate ................................................................5.0mg
Nicotinamide..............................................................................5.0mg
Riboflavin ..................................................................................5.0mg
Thiamine·HCl ............................................................................5.0mg
Biotin .........................................................................................2.0mg
Folic acid....................................................................................2.0mg
Cyanocobalamin ........................................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize and add aseptically to sterile basal medium.
Preparation of Medium:  Add components—except Metals “44”,
phosphate solution, and vitamin solution—to distilled/deionized water
and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Adjust pH of basal medium to 7.0. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 50°C and aseptically add the Metals “44”,
phosphate, and vitamin solutions.
Use: For the cultivation and maintenance of Halomonas meridiana
and other Halomonas species.