AOAC Letheen Broth (Association of Official Analytical Chemists Letheen Broth)

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山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

AOAC Letheen Broth
(Association of Official Analytical Chemists
Letheen Broth)
Composition per liter:
Peptic digest of animal tissue......................................................10.0g
Polysorbate 80...............................................................................5.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................5.0g
Lecithin.........................................................................................0.7g
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes in 10.0mL volumes. Autoclave for 15
min at 15 psi pressure–121°C.
Use: For the determination of phenol coefficients of disinfectant prod-
ucts containing cationic surface-active materials. Use according to
Official Methods of Analysis of the Association of Official Analytical
Chemists (AOAC).