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Yeast Fermentation Medium

[所属分类:培养基配方] [发布时间:2021-12-29] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone ...............................................................7.5g

Yeast extract........................................................4.5g

Bromthymol Blue (1.6% solution) ................. 1.0mL

Carbohydrate solution..................................... 1.0mL

Carbohydrate Solution:

Composition per 10.0mL:

Carbohydrate.......................................................0.6g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 10.0mL. Glucose, maltose, lactose, galac

tose, or trehalose may be used. If raffinose is used, pre

pare a 12% solution. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 1.0L. Mix thoroughly. Gently

heat and bring to boiling. Distribute in 2.0mL vol

umes into test tubes that contain an inverted Durham

tube. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 45°–50°C. Aseptically add 1.0mL of sterile

carbohydrate solution. Mix thoroughly.

Use: For the cultivation and differentiation of yeast

based on carbohydrate fermentation patterns. Yeasts

that can ferment a specific carbohydrate turn the medi

um yellow.