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培养基配方
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YC Medium without Tryptophan
[所属分类:培养基配方] [发布时间:2021-12-29] [发布人:网站管理员2] [阅读次数:] [返回]
YC Medium without Tryptophan
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Solution A:
Composition per 500.0mL:
Glucose .............................................................20.0g
Succinic acid.....................................................10.0g
NaOH..................................................................6.0g
(NH4)2SO4...........................................................5.0g
KH2PO4...............................................................1.0g
MgSO4·7H2O ......................................................0.5g
NaCl....................................................................0.1g
CaCl2·2H2O.........................................................0.1g
Adenine...............................................................0.1g
L-Arginine...........................................................0.1g
L-Cysteine ...........................................................0.1g
L-Leucine ............................................................0.1g
L-Lysine ..............................................................0.1g
L-Threonine.........................................................0.1g
Uracil ..................................................................0.1g
L-Aspartic acid..................................................0.05g
L-Histidine ........................................................0.05g
L-Isoleucine.......................................................0.05g
L-Methionine.....................................................0.05g
L-Phenylalanine ................................................0.05g
L-Proline............................................................0.05g
L-Serine.............................................................0.05g
L-Tyrosine .........................................................0.05g
L-Valine.............................................................0.05g
Inositol .............................................................2.0mg
KI .....................................................................1.0mg
H3BO3 ..............................................................0.5mg
ZnSO4·7H2O ....................................................0.4mg
MnSO4·4H2O ...................................................0.4mg
Thiamine·HCl ..................................................0.4mg
Pyroxidine·HCl ................................................0.4mg
Niacin...............................................................0.4mg
Calcium pantothenate ......................................0.4mg
p-Aminobenzoic acid.......................................0.2mg
Riboflavin ........................................................0.2mg
FeCl3 ................................................................0.2mg
Na2MoO4·4H2O ...............................................0.2mg
CuSO4·5H2O ..................................................0.04mg
Folic acid.......................................................... 2.0μg
Biotin ............................................................... 2.0μg
Preparation of Solution A: Add components to
distilled/deionized water and bring volume to
500.0mL. Adjust pH to 5.6–5.7. Filter sterilize.
Warm to 50°–55°C.
Solution B:
Composition per 500.0mL:
Agar, noble........................................................20.0g
Preparation of Solution B: Add agar to distilled/
deionized water and bring volume to 500.0mL. Gen
tly heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C.Cool to 50–55°C.
Preparation of Medium: Aseptically combine
500.0mL of solution A and 500.0mL of solution B.
Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the cultivation of Saccharomyces cerevisiae.
