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YC Agar without Tryptophan
[所属分类:培养基配方] [发布时间:2021-12-29] [发布人:网站管理员2] [阅读次数:] [返回]
YC Agar without Tryptophan
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Solution A................................................... 500.0mL
Solution B ................................................... 500.0mL
Solution A:
Composition per 500.0mL:
Glucose .............................................................20.0g
Succinic acid.....................................................10.0g
NaOH..................................................................6.0g
(NH4)2SO4 ..........................................................5.0g
KH2PO4...............................................................0.6g
MgSO4·7H2O ......................................................0.3g
Adenine...............................................................0.1g
L-Arginine...........................................................0.1g
L-Cysteine ...........................................................0.1g
L-Leucine ............................................................0.1g
L-Lysine ..............................................................0.1g
L-Threonine.........................................................0.1g
Uracil ..................................................................0.1g
L-Aspartic acid..................................................0.05g
L-Histidine ........................................................0.05g
L-Isoleucine.......................................................0.05g
L-Methionine.....................................................0.05g
L-Phenylalanine ................................................0.05g
L-Proline ...........................................................0.05g
L-Serine.............................................................0.05g
L-Tyrosine .........................................................0.05g
L-Valine.............................................................0.05g
NaCl..................................................................0.05g
CaCl2·2H2O ......................................................0.05g
DL-Methionine...................................................0.01g
L-Histidine·HCl ...............................................0.005g
Inositol .............................................................1.0mg
KI .....................................................................0.5mg
H3BO3 ..............................................................0.3mg
ZnSO4·7H2O ....................................................0.2mg
MnSO4·4H2O...................................................0.2mg
Thiamine·HCl ..................................................0.2mg
Pyroxidine·HCl ................................................0.2mg
Niacin...............................................................0.2mg
Calcium pantothenate ......................................0.2mg
p-Aminobenzoic acid.......................................0.1mg
Riboflavin ........................................................0.1mg
FeCl3 ................................................................0.1mg
Na2MoO4·4H2O ...............................................0.1mg
CuSO4·5H2O..................................................0.02mg
Folic acid ..........................................................1.0μg
Biotin ................................................................1.0μg
pH 5.7 ± 0.2 at 25°C
Preparation of Solution A: Add components to
distilled/deionized water and bring volume to
500.0mL. Mix thoroughly. Adjust pH to 5.6–5.7. Fil
ter sterilize. Warm to 50°–55°C.
Solution B:
Composition per 500.0mL:
Agar, noble........................................................20.0g
Preparation of Solution B: Add agar to distilled/
deionized water and bring volume to 500.0mL. Mix
thoroughly. Gently heat and bring to boiling. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to
50°–55°C.
Preparation of Medium: Aseptically mix
500.0mL of solution A and 500.0mL of solution B.
Mix thoroughly. Pour into sterile Petri dishes or dis
tribute into sterile tubes.
Use: For the cultivation and maintenance of Saccharo
myces cerevisiae.
