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Phenylalanine Agar (Phenylalanine Deaminase Medium) (BAM M123)

[所属分类:培养基配方] [发布时间:2021-12-9] [发布人:网站管理员2] [阅读次数:] [返回]

Phenylalanine Agar

(Phenylalanine Deaminase Medium)

(BAM M123)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................12.0g

NaCl....................................................................5.0g

Yeast extract........................................................3.0g

L-Phenylalanine ..................................................1.0g

Na2HPO4.............................................................1.0g

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Distribute into tubes or flasks. Autoclave

for 10 min at 15 psi pressure–121°C. Pour into sterile

Petri dishes or leave in tubes.

Use: For the differentiation of enteric Gram-nega

tive bacilli on the basis of their ability to produce

phenylpyruvic acid from phenylalanine. After appro-

priate incubation of bacteria, ferric chloride reagent

is added on the agar. Formation of a green color in 1–

5 min indicates the production of phenylpyruvic acid.