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Phenylalanine Agar (Phenylalanine Deaminase Medium) (BAM M123)

[所属分类:培养基配方] [发布时间:2021-12-9] [发布人:网站管理员2] [阅读次数:] [返回]

Phenylalanine Agar

(Phenylalanine Deaminase Medium)

(BAM M123)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................12.0g

NaCl....................................................................5.0g

Yeast extract........................................................3.0g

DL-Phenylalanine ................................................2.0g

Na2HPO4.............................................................1.0g

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Distribute into tubes or flasks. Autoclave

for 10 min at 15 psi pressure–121°C. Pour into sterile

Petri dishes or leave in tubes.

Use: For the differentiation of enteric Gram-nega

tive bacilli on the basis of their ability to produce

phenylpyruvic acid from phenylalanine. After appro

priate incubation of bacteria, ferric chloride reagent

is added on the agar. Formation of a green color in 1–

5 min indicates the production of phenylpyruvic acid.