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Phenol Red Mannitol Agar

[所属分类:培养基配方] [发布时间:2021-12-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Mannitol............................................................10.0g

Proteose peptone No. 3.....................................10.0g

NaCl....................................................................5.0g

Beef extract.........................................................1.0g

Phenol Red.....................................................25.0mg

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Distrib

ute into tubes or flasks. Autoclave for 15 min at 13 psi

pressure–118°C. Pour into sterile Petri dishes or leave

in tubes. Allow tubes to cool in a slanted position.

Use: For determination of the ability of a microor

ganism to ferment mannitol. Fermentation is deter

mined by the production of acid—medium turns

yellow.