培养基配方

您现在的位置：网站首页 >> 培养基配方

Phenol Red Lactose Broth

[所属分类:培养基配方] [发布时间:2021-12-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................10.0g

Lactose................................................................5.0g

NaCl....................................................................5.0g

Phenol Red......................................................0.018g

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Adjust pH to 7.3 if necessary. Dis

tribute into tubes containing an inverted Durham

tube. Fill each tube with 10.0mL of medium. Auto

clave for 15 min at 13 psi pressure–118°C.

Use: For determination of the ability of a microor

ganism to ferment lactose. Fermentation is deter

mined by the production of acid—broth turns

yellow—and formation of gas—bubble trapped in

Durham tube.