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Phenol Red Broth

[所属分类:培养基配方] [发布时间:2021-12-9] [发布人:网站管理员2] [阅读次数:] [返回]

Phenol Red Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................10.0g

NaCl....................................................................5.0g

Phenol Red......................................................0.018g

Carbohydrate solution................................... 20.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Carbohydrate Solution:

Composition per 20.0mL:

Carbohydrate..................................... 5.0–10.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 20.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 980.0mL. Mix thoroughly.

Adjust pH to 7.4 if necessary. Distribute into tubes

containing an inverted Durham tube. Fill each tube

with 9.8mL of medium. Autoclave for 15 min at 13 psi

pressure–118°C. Cool to 45°–50°C. Aseptically add

0.2mL of sterile carbohydrate solution to each tube.

Use: For the determination of fermentation reactions

in the differentiation of microorganisms. Fermenta

tion is determined by the production of acid—broth

turns yellow—and formation of gas—bubble trapped

in Durham tube.