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Phenol Red Agar

[所属分类:培养基配方] [发布时间:2021-12-9] [发布人:网站管理员2] [阅读次数:] [返回]

Phenol Red Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Proteose peptone No. 3.....................................10.0g

NaCl....................................................................5.0g

Beef extract.........................................................1.0g

Phenol Red......................................................0.025g

Carbohydrate solution................................... 20.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Carbohydrate Solution:

Composition per 20.0mL:

Carbohydrate..............................................5.0–10.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 20.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 980.0mL. Mix thoroughly.

Adjust pH to 7.4 if necessary. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti

cally add 20.0mL of sterile carbohydrate solution.

Pour into sterile Petri dishes or distribute into sterile

tubes. Allow tubes to cool in a slanted position.

Use: For the determination of fermentation reac

tions. Bacteria that can ferment the added carbohy

drate turn the medium yellow.