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PALCAM Agar (Polymyxin Acriflavine LiCl Ceftazidime Esculin Mannitol Agar) (PALCAM Listeria Selective Agar) (BAM M118a)

[所属分类:培养基配方] [发布时间:2021-12-8] [发布人:网站管理员2] [阅读次数:] [返回]

PALCAM Agar

(Polymyxin Acriflavine LiCl

Ceftazidime Esculin Mannitol Agar)

(PALCAM Listeria Selective Agar)

(BAM M118a)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone .............................................................23.0g

LiCl...................................................................15.0g

Agar ..................................................................10.0g

Mannitol............................................................10.0g

NaCl....................................................................5.0g

Yeast extract........................................................3.0g

Starch ..................................................................1.0g

Esculin ................................................................0.8g

Ferric ammonium citrate.....................................0.5g

Glucose ...............................................................0.5g

Phenol Red........................................................0.08g

PALCAM selective supplement.................... 10.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

PALCAM Selective Supplement:

Composition per 10.0mL:

Ceftazidime....................................................20.0mg

Polymyxin B ..................................................10.0mg

Acriflavine·HCl................................................5.0mg

Preparation of PALCAM Selective Supple

ment: Add components to distilled/deionized water

and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Egg Yolk Emulsion:

Composition:

Chicken egg yolks..................................................11

Whole chicken egg...................................................1

Preparation of Egg Yolk Emulsion: Soak eggs

with 1:100 dilution of saturated mercuric chloride so

lution for 1 min. Crack eggs and separate yolks from

whites. Mix egg yolks with 1 chicken egg.

Preparation of Medium: Add components, ex

cept PALCAM selective supplement, to distilled/

deionized water and bring volume to 990.0mL. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add sterile PALCAM selec

tive supplement. Mix thoroughly. The addition of

25.0mL of egg yolk emulsion may aid in the recovery

of damaged Listeria. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the selective isolation, cultivation, and dif

ferentiation of Listeria monocytogenes and other

Listeria species from foods.

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PALCAM Agar (Polymyxin Acriflavine LiCl Ceftazidime Esculin Mannitol Agar) (PALCAM Listeria Selective Agar) (BAM M118a)