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Pai Medium

[所属分类:培养基配方] [发布时间:2021-12-8] [发布人:网站管理员2] [阅读次数:] [返回]

Pai Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Homogenized whole egg ............................ 666.0mL

NaCl (0.85% solution)................................ 334.0mL

pH 6.75 ± 0.2 at 25°C

Homogenized Whole Egg:

Composition per liter:

Whole eggs ...................................................... 18-24

Preparation of Homogenized Whole Egg: Use

fresh eggs, less than 1 week old. Scrub the shells with

soap. Let stand in a soap solution for 30 min. Rinse in

running water. Soak eggs in 70% ethanol for 15 min.

Break the eggs into a sterile container. Homogenize by

shaking. Filter through four layers of sterile cheesecloth

into a sterile graduated cylinder. Measure out 1.0L.

Preparation of Medium: Combine components.

Mix thoroughly. Aseptically distribute into sterile

tubes. Inspissate tubes in a slanted position at 80°–

90°C (moist heat) for 30 min.

Use: For the maintenance of stock cultures of Salmo

nella typhi and other Salmonella species.