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M9 Medium with Arginine

[所属分类:培养基配方] [发布时间:2021-11-30] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1013.0mL:

L-Proline.........................................................20.0mg

L-Arginine......................................................20.0mg

10X M9 salts............................................... 100.0mL

Glucose solution ........................................... 10.0mL

CaCl2·2H2O solution....................................... 1.0mL

MgSO4 solution............................................... 1.0mL

Thiamine·HCl solution ................................... 1.0mL

pH 7.4 ± 0.2 at 25°C

10X M9 Salts:

Composition per liter:

Na2HPO4...........................................................60.0g

KH2PO4.............................................................30.0g

NH4Cl ...............................................................10.0g

NaCl....................................................................5.0g

Preparation of 10X M9 Salts: Add components

to distilled/deionized water and bring volume to

1.0L. Mix thoroughly. Adjust pH to 7.4.

Glucose Solution:

Composition per 100.0mL:

Glucose .............................................................20.0g

Preparation of Glucose Solution: Add glucose

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

CaCl2·2H2O Solution:

Composition per 100.0mL:

CaCl2·2H2O ......................................................1.47g

Preparation of CaCl2·2H2O Solution: Add

CaCl2·2H2O to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Autoclave for

15 min at 15 psi pressure–121°C.

MgSO4 Solution:

Composition per 100.0mL:

MgSO4 ............................................................12.04g

Preparation of MgSO4 Solution: Add MgSO4

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C.

Thiamine·HCl Solution:

Composition per 10.0mL:

Thiamine·HCl ...................................................3.37g

Preparation of Thiamine·HCl Solution: Add

thiamine·HCl to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add L-proline, L-argin

ine, and 10X M9 salts to distilled/deionized water

and bring volume to 1.0L. Mix thoroughly. Auto

clave for 15 min at 15 psi pressure–121°C. Aseptical

ly add 10.0mL of sterile glucose solution, 1.0mL of

sterile CaCl2·2H2O solution, 1.0mL of sterile MgSO4

solution, and 1.0mL of sterile thiamine·HCl solution.

Mix thoroughly. Aseptically distribute into sterile

tubes or flasks.

Use: For the cultivation of Escherichia coli.

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M9 Medium with Arginine