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M17 Medium for Lactic Streptococci (DSMZ Medium 449)

[所属分类:培养基配方] [发布时间:2021-11-30] [发布人:网站管理员2] [阅读次数:] [返回]

M17 Medium for Lactic Streptococci

(DSMZ Medium 449)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2-ß-glycerophosphate ...................................19.0g

Peptone from casein............................................5.0g

Soya peptone.......................................................5.0g

Peptone bacteriological.......................................5.0g

Yeast extract........................................................2.5g

Ascorbic acid ......................................................0.5g

MgSO4·7H2O....................................................0.25g

Lactose solution............................................ 10.0mL

pH 6.9 ± 0.2 at 25°C

Lactose Solution:

Composition per 10.0mL:

Lactose................................................................5.0g

Preparation of Lactose Solution: Add lactose

to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept lactose solution, to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 25°C.

Aseptically add 10.0mL sterile lactose solution.

Aseptically distribute to sterile tubes or flasks.

Use: For the cultivation and maintenance of Lactococ

cus lactis subsp. lactis=Streptococcus lactis.