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Lysine Arginine Iron Agar (LAIA) (BAM M86)

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

Lysine Arginine Iron Agar

(LAIA)

(BAM M86)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

L-Arginine .........................................................10.0g

L-Lysine.............................................................10.0g

Peptone................................................................5.0g

Yeast extract........................................................3.0g

Glucose ...............................................................1.0g

Ferric ammonium citrate.....................................0.5g

Sodium thiosulfate ............................................0.04g

Bromcresol Purple ............................................0.02g

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Adjust pH

to 6.8. Distribute into screw-capped tubes in 5.0mL

volumes. Autoclave for 12 min at 15 psi pressure–

121°C. Allow tubes to cool in a slanted position.

Use: For the cultivation and differentiation of bacteria

based on their ability to decarboxylate lysine, decarbox

ylate arginine, and produce H2S. Bacteria that decar

boxylate lysine or arginine turn the medium purple.

Bacteria that produce H2S appear as black colonies.