培养基配方

您现在的位置：网站首页 >> 培养基配方

Lysine Agar, Selective

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

L-Lysine.............................................................10.0g

Peptone ...............................................................5.0g

Glucose ...............................................................3.5g

Yeast extract........................................................3.0g

Bile salts mixture ................................................1.5g

Sulfapyridine.......................................................0.3g

Bromcresol Purple ............................................0.03g

Crystal Violet..................................................0.001g

pH 6.8 ± 0.1 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the selective isolation and cultivation of

Salmonella species from food by the hydrophobic

grid membrane filter method.