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LMX Broth Modified, Fluorocult (Fluorocult LMX Broth, Modified)

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

LMX Broth Modified, Fluorocult

(Fluorocult LMX Broth, Modified)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tryptose ................................................................5.0

NaCl......................................................................5.0

K2HPO4...............................................................2.7g

KH2PO4...............................................................2.0g

Sorbitol..................................................................1.0

Tryptophan............................................................1.0

Lauryl sulfate sodium salt.....................................0.1

5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside

(X-GAL).......................................................0.08

4-Methylumbelliferyl-β-D-glucuronide ............0.05g

1-Isopropyl-β-D-1-thio-galactopyranoside

(IPTG) ............................................................0.1

pH: 6.8 ± 0.2 at 25°C

Source: This medium is available from Merck.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Distribute into test tubes. Autoclave for 15

min at 15 psi pressure–121°C. The broth is clear and

yellowish brown.

Use: For the simultaneous detection of total

coliforms and E. coli in water, food and dairy prod

ucts by the fluorogenic procedure. A color change of

the broth from yellow to blue-green indicates the

presence of coliforms. A blue fluorescence under

long-wave UV light permits the rapid detection of E.

coli. As tryptophan is added to the broth, the indole

reaction is easily done by adding KOVACS reagent.

The formation of a red ring additionally confirms the

presence of E. coli.